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Illumina Inc
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Illumina Inc
guide rna cassette ![]() Guide Rna Cassette, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/guide rna cassette/product/Illumina Inc Average 90 stars, based on 1 article reviews
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Synthetic Genomics Inc
v. natriegens vmaxtm express, δ dns, insertion of iptg-inducible t7 rna polymerase cassette ![]() V. Natriegens Vmaxtm Express, δ Dns, Insertion Of Iptg Inducible T7 Rna Polymerase Cassette, supplied by Synthetic Genomics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/v. natriegens vmaxtm express, δ dns, insertion of iptg-inducible t7 rna polymerase cassette/product/Synthetic Genomics Inc Average 90 stars, based on 1 article reviews
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GenScript corporation
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Sangon Biotech
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Promega
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Image Search Results
Journal: Frontiers in Plant Science
Article Title: Evaluate the guide RNA effectiveness via Agrobacterium -mediated transient assays in Nicotiana benthamiana
doi: 10.3389/fpls.2023.1111683
Figure Lengend Snippet: The pgRNA-YFP vectors are used to screen the effectiveness of gRNAs in Agrobacterium -mediated transient assays. Transient expression of pgRNA-YFP alone in N. benthamiana does not produce any fluorescent signals. (A) Co-expression of p NbEDS1 gRNA-YFP with pCas9- NbNDR/NbEDS1 gRNA restored the YFP signals in N. benthamiana . (B) Co-expression of p NbNDR1 gRNA-YFP with pCas9- NbNDR1/NbEDS1 gRNA failed to restore the YFP signals. (C) Co-expression of p NbWRKY70 gRNA-YFP with pCas9- NbWRKY70 gRNA restored the YFP signals. (D, E) Co-expression of either p GmKTI1 gRNA-YFP or p GmKTI3 gRNA-YFP with pCas9- GmKTI1/GmKTI3 gRNA restored the YFP signals in N. benthamiana . Scale bar represents 100 µm.
Article Snippet: The
Techniques: Expressing
Journal: Frontiers in Plant Science
Article Title: Evaluate the guide RNA effectiveness via Agrobacterium -mediated transient assays in Nicotiana benthamiana
doi: 10.3389/fpls.2023.1111683
Figure Lengend Snippet: Sanger sequencing chromatograms display the DNA sequence of GmKTI1 and GmKTI3 that is targeted for gene editing. The open reading frames of GmKTI1 and GmKTI3 in the pCas9-GmKTI1/GmKTI3 gRNA T0 transgenic soybean plants were amplified and sequenced. The double peaks in the sequencing chromatograms indicate the presence of both wild-type and mutant allele of sequences GmKTI1 (A) and GmKTI3 (B) . The wild-type gene sequences are shown on the top of the sequencing chromatograms. The PAM sites are underlined.
Article Snippet: The
Techniques: Sequencing, Transgenic Assay, Amplification, Mutagenesis